PrimePCR™ Probe Assay: CAV3, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024961
Assay Design:   Exonic
Chromosome Location:   3:8787824-8787968question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000343849 ENST00000397368

Gene Information

This gene encodes a caveolin family member which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C) hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus with inclusion or exclusion of a differentially spliced intron. In addition transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq Jul 2008]

Gene Symbol:   CAV3
Gene Name:   caveolin 3
Aliases:   LGMD1C, LQT9, MGC126100, MGC126101, MGC126129, VIP-21, VIP21
RefSeq:   NC_000003.11 NT_022517.18 NG_008797.1
Ensembl:   ENSG00000182533
Entrez:   859
UniGene:   Hs.98303
Chromosome Mapping:   3p25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.690000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download