This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CAV3, Human
PrimePCR™ Template for Probe Assay: CAV3, Human
This gene encodes a caveolin family member which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C) hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus with inclusion or exclusion of a differentially spliced intron. In addition transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.