This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PTX4, Human
PrimePCR™ Template for Probe Assay: PTX4, Human
PTX4 belongs to the pentraxin (PTX) superfamily of multifunctional conserved proteins. Pentraxins are characterized by a cyclic multimeric structure and a conserved C-terminal pentraxin domain of approximately 200 amino acids. All pentraxins have a conserved 8-amino acid sequence HxCxS/TWxS in which x is any amino acid within their pentraxin domain. Pentraxins are divided into short proteins such as C-reactive protein (CRP; MIM 123260) and serum amyloid P protein (APCS; MIM 104770) and long proteins such as PTX3 (MIM 602492) and PTX4. Some PTXs are part of the humoral arm of innate immunity and behave as functional ancestors of antibodies by mediating agglutination complement activation and opsonization (summary by Martinez de la Torre et al. 2010 [PubMed 20357257]).[supplied by OMIM Jun 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.