PrimePCR™ Probe Assay: C22orf25, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024948
Assay Design:   Exonic
Chromosome Location:   22:20052065-20052171question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000401886 ENST00000398042 ENST00000327374 ENST00000401833 ENST00000447208 ENST00000432883 ENST00000434570 ENST00000456048 ENST00000420290

Gene Information

Description Not Available

Gene Symbol:   C22orf25
Gene Name:   chromosome 22 open reading frame 25
Aliases:   DKFZp761P1121
RefSeq:   NC_000022.10 NG_023326.1 NT_011519.10
Ensembl:   ENSG00000183597
Entrez:   128989
UniGene:   Hs.474233
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.000000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download