PrimePCR™ Probe Assay: TGIF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024947
Assay Design:   Exonic
Chromosome Location:   18:3456425-3456538question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000552383 ENST00000550958 ENST00000548489 ENST00000549780 ENST00000549253 ENST00000405385 ENST00000343820 ENST00000407501 ENST00000546979 ENST00000551402 ENST00000551541 ENST00000345133 ENST00000330513 ENST00000549546 ENST00000549468 ENST00000551333 ENST00000472042 ENST00000401449 ENST00000400167

Gene Information

The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4 which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants encoding four distinct isoforms are described. [provided by RefSeq Jul 2008]

Gene Symbol:   TGIF1
Gene Name:   TGFB-induced factor homeobox 1
Aliases:   HPE4, MGC39747, MGC5066, TGIF
RefSeq:   NC_000018.9 NT_010859.14 NG_007447.1
Ensembl:   ENSG00000177426
Entrez:   7050
UniGene:   Hs.373550
Chromosome Mapping:   18p11.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.988100
y-intercept 37.540000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download