PrimePCR™ Probe Assay: CDK14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDK14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDK14, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024924
Assay Design:   Exonic
Chromosome Location:   7:90355948-90356090question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000449528 ENST00000446224 ENST00000430760 ENST00000456689 ENST00000380050 ENST00000446790 ENST00000265741 ENST00000406263 ENST00000436577

Gene Information

PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen 2001 [PubMed 11313143]).[supplied by OMIM Mar 2008]

Gene Symbol:   CDK14
Gene Name:   cyclin-dependent kinase 14
Aliases:   KIAA0834, PFTAIRE1, PFTK1
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000058091
Entrez:   5218
Chromosome Mapping:   7q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.890000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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