PrimePCR™ Probe Assay: HARBI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024912
Assay Design:   Exonic
Chromosome Location:   11:46637676-46637825question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000326737 ENST00000529192 ENST00000532281

Gene Information

Description Not Available

Gene Symbol:   HARBI1
Gene Name:   harbinger transposase derived 1
Aliases:   C11orf77, FLJ32675
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000180423
Entrez:   283254
UniGene:   Hs.714463
Chromosome Mapping:   11p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.400000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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