This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: BTN3A2, Human
PrimePCR™ Template for Probe Assay: BTN3A2, Human
This gene encodes a member of the butyrophilin (BTN) family and the immunoglobulin (IG) superfamily. The gene resides in the juxta-telomeric region of the major histocompatability class 1 locus and is clustered with the other six family members including BTN BTN2A1 BTN2A2 BTN2A3 BTN3A1 and BTN3A3 on chromosome 6. The full-length protein encoded by this gene is a type I membrane protein. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.