This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: RAP1GAP, Human
PrimePCR™ Template for Probe Assay: RAP1GAP, Human
This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene RAP1GAP promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins guanine nucleotide exchange factors (GEFs) act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general ras subfamily proteins such as RAP1 play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation adhesion differentiation and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.