This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CTLA4, Human
PrimePCR™ Template for Probe Assay: CTLA4, Human
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain a transmembrane domain and a cytoplasmic tail. Alternate transcriptional splice variants encoding different isoforms have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus Graves disease Hashimoto thyroiditis celiac disease systemic lupus erythematosus thyroid-associated orbitopathy and other autoimmune diseases. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.