PrimePCR™ Probe Assay: ASIP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024697
Assay Design:   Exonic
Chromosome Location:   20:32848220-32850641question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000374954

Gene Information

In mice the agouti gene encodes a paracrine signaling molecule that causes hair follicle melanocytes to synthesize pheomelanin a yellow pigment instead of the black or brown pigment eumelanin. Pleiotropic effects of constitutive expression of the mouse gene include adult-onset obesity increased tumor susceptibility and premature infertility. This gene is highly similar to the mouse gene and encodes a secreted protein that may (1) affect the quality of hair pigmentation (2) act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone (3) play a role in neuroendocrine aspects of melanocortin action and (4) have a functional role in regulating lipid metabolism in adipocytes. [provided by RefSeq Jul 2008]

Gene Symbol:   ASIP
Gene Name:   agouti signaling protein
Aliases:   AGSW, AGTI, AGTIL, ASP, MGC126092, MGC126093, SHEP9
RefSeq:   NC_000020.10 NG_011439.1 NT_011362.10
Ensembl:   ENSG00000101440
Entrez:   434
UniGene:   Hs.659995
Chromosome Mapping:   20q11.2-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.920000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download