This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ASIP, Human
PrimePCR™ Template for Probe Assay: ASIP, Human
In mice the agouti gene encodes a paracrine signaling molecule that causes hair follicle melanocytes to synthesize pheomelanin a yellow pigment instead of the black or brown pigment eumelanin. Pleiotropic effects of constitutive expression of the mouse gene include adult-onset obesity increased tumor susceptibility and premature infertility. This gene is highly similar to the mouse gene and encodes a secreted protein that may (1) affect the quality of hair pigmentation (2) act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone (3) play a role in neuroendocrine aspects of melanocortin action and (4) have a functional role in regulating lipid metabolism in adipocytes. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.