PrimePCR™ Probe Assay: UQCR10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024653
Assay Design:   Exonic
Chromosome Location:   22:30165667-30165824question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000330029 ENST00000401406 ENST00000332801 ENST00000406782

Gene Information

UCRC is a subunit of mitochondrial complex III (ubiquinol-cytochrome c reductase; EC 1.10.2.2) which forms the middle segment of the respiratory chain of the inner mitochondrial membrane (Schagger et al. 1995 [PubMed 8592474]).[supplied by OMIM Mar 2008]

Gene Symbol:   UQCR10
Gene Name:   ubiquinol-cytochrome c reductase, complex III subunit X
Aliases:   HSPC051, HSPC151, QCR9, UCCR7.2, UCRC
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000184076
Entrez:   29796
UniGene:   Hs.284292
Chromosome Mapping:   22cen-q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.060000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download