This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: UQCR10, Human
PrimePCR™ Template for Probe Assay: UQCR10, Human
UCRC is a subunit of mitochondrial complex III (ubiquinol-cytochrome c reductase; EC 22.214.171.124) which forms the middle segment of the respiratory chain of the inner mitochondrial membrane (Schagger et al. 1995 [PubMed 8592474]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.