This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SLC35B2, Human
PrimePCR™ Template for Probe Assay: SLC35B2, Human
Sulfotransferases (e.g. SULT4A1; MIM 608359) use an activated form of sulfate 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) as a common sulfate donor for sulfation of glycoproteins proteoglycans and glycolipids in the endoplasmic reticulum and Golgi apparatus. SLC35B2 is located in the microsomal membrane and transports PAPS from the cytosol where it is synthesized into the Golgi lumen (Kamiyama et al. 2003 [PubMed 12716889]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.