PrimePCR™ Probe Assay: HTR1E, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024593
Assay Design:   Exonic
Chromosome Location:   6:87725201-87725329question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000369584 ENST00000305344

Gene Information

Description Not Available

Gene Symbol:   HTR1E
Gene Name:   5-hydroxytryptamine (serotonin) receptor 1E
Aliases:   5-HT1E
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000168830
Entrez:   3354
UniGene:   Hs.1611
Chromosome Mapping:   6q14-q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.980000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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