This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SH3BP4, Human
PrimePCR™ Template for Probe Assay: SH3BP4, Human
This gene encodes a protein with 3 Asn-Pro-Phe (NPF) motifs an SH3 domain a PXXP motif a bipartite nuclear targeting signal and a tyrosine phosphorylation site. This protein is involved in cargo-specific control of clathrin-mediated endocytosis specifically controlling the internalization of a specific protein receptor. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.