PrimePCR™ Probe Assay: SLC22A18, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC22A18, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC22A18, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024342
Assay Design:   Exonic
Chromosome Location:   11:2943676-2943776question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000347936 ENST00000312221 ENST00000449793 ENST00000380574

Gene Information

This gene is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of 11p15.5 an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocortical carcinoma and lung ovarian and breast cancer. This gene is imprinted with preferential expression from the maternal allele. Mutations in this gene have been found in Wilms' tumor and lung cancer. This protein may act as a transporter of organic cations and have a role in the transport of chloroquine and quinidine-related compounds in kidney. Two alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq Oct 2010]

Gene Symbol:   SLC22A18
Gene Name:   solute carrier family 22, member 18
Aliases:   BWR1A, BWSCR1A, DKFZp667A184, HET, IMPT1, ITM, ORCTL2, SLC22A1L, TSSC5, p45-BWR1A
RefSeq:   NC_000011.9 NT_009237.18 NG_011512.1
Ensembl:   ENSG00000110628
Entrez:   5002
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996500
y-intercept 36.010000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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