This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: UPP1, Human
PrimePCR™ Template for Probe Assay: UPP1, Human
The 2 known types of pyrimidine nucleoside phosphorylases uridine phosphorylase (UP; EC 184.108.40.206) and thymidine phosphorylase (TP; EC 220.127.116.11) in the presence of orthophosphate catalyze the reversible phosphorolysis of uridine and thymidine or deoxyuridine respectively to free bases and ribose-1-phosphate or deoxyribose-1-phosphate. Pyrimidine nucleoside phosphorylases can add ribose or deoxyribose to pyrimidine bases to form nucleosides that can be incorporated into RNA or DNA (Watanabe and Uchida 1995 [PubMed 7488099]).[supplied by OMIM Aug 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.