PrimePCR™ Probe Assay: NKX6-3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024274
Assay Design:   Exonic
Chromosome Location:   8:41507780-41507944question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000518699 ENST00000425142

Gene Information

The NKX family of homeodomain proteins controls numerous developmental processes. Members of the NKX6 subfamily including NKX6-3 are involved in development of the central nervous system (CNS) gastrointestinal tract and pancreas (Alanentalo et al. 2006 [PubMed 16326147]).[supplied by OMIM Mar 2008]

Gene Symbol:   NKX6-3
Gene Name:   NK6 homeobox 3
Aliases:   FLJ25169, NKX6.3
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000165066
Entrez:   157848
UniGene:   Hs.647132
Chromosome Mapping:   8p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.450000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download