PrimePCR™ Probe Assay: ARID4A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ARID4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARID4A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024264
Assay Design:   Exonic
Chromosome Location:   14:58831785-58831960question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000355431 ENST00000395168 ENST00000431317 ENST00000417477 ENST00000348476

Gene Information

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly with several other proteins to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein in turn serves as a bridging molecule to recruit HDACs and in addition provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity. Multiple alternatively spliced transcripts have been observed for this gene although not all transcript variants have been fully described. [provided by RefSeq Jul 2008]

Gene Symbol:   ARID4A
Gene Name:   AT rich interactive domain 4A (RBP1-like)
Aliases:   RBBP-1, RBBP1, RBP-1, RBP1
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000032219
Entrez:   5926
Chromosome Mapping:   14q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.560000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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