PrimePCR™ Probe Assay: DAK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024262
Assay Design:   Exonic
Chromosome Location:   11:61113823-61113980question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000394900 ENST00000529479

Gene Information

This gene is a member of the family of dihydroxyacetone kinases which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone and also catalyzes the formation of riboflavin 4'5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified but the full-length nature of only one has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DAK
Gene Name:   dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Aliases:   DKFZp586B1621, MGC5621, NET45
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000149476
Entrez:   26007
UniGene:   Hs.6278
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.420000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download