PrimePCR™ Probe Assay: LINC00637, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024180
Assay Design:   Exonic
Chromosome Location:   14:104323854-104324030question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000556586

Gene Information

Not Available

Gene Symbol:   LINC00637
Gene Name:   long intergenic non-protein coding RNA 637 [Source:HGNC Symbol;Acc:HGNC:27069]
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000258735
Entrez:   145216
UniGene:   Hs.578091
Chromosome Mapping:   14q32.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.460000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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