PrimePCR™ Probe Assay: ZBTB38, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024118
Assay Design:   Exonic
Chromosome Location:   3:141161317-141161450question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000509842 ENST00000507722 ENST00000513258 ENST00000509883 ENST00000514251 ENST00000510338 ENST00000504673 ENST00000513570 ENST00000441582 ENST00000510726 ENST00000509813 ENST00000321464

Gene Information

The protein encoded by this gene is a zinc finger transcriptional activator that binds methylated DNA. The encoded protein can form homodimers or heterodimers through the zinc finger domains. In mouse inhibition of this protein has been associated with apoptosis in some cell types. [provided by RefSeq Jun 2010]

Gene Symbol:   ZBTB38
Gene Name:   zinc finger and BTB domain containing 38
Aliases:   CIBZ, FLJ22332, FLJ31131, FLJ35036, ZNF921
RefSeq:   NC_000003.11 NG_021426.1 NT_005612.16
Ensembl:   ENSG00000177311
Entrez:   253461
UniGene:   Hs.730645
Chromosome Mapping:   3q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.890000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download