PrimePCR™ Probe Assay: DOLK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024093
Assay Design:   Exonic
Chromosome Location:   9:131709015-131709133question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000372586 ENST00000515348

Gene Information

The protein encoded by this gene catalyzes the CTP-mediated phosphorylation of dolichol and is involved in the synthesis of Dol-P-Man which is an essential glycosyl carrier lipid for C- and O-mannosylation N- and O-linked glycosylation of proteins and for the biosynthesis of glycosyl phosphatidylinositol anchors in endoplasmic reticulum. Mutations in this gene are associated with dolichol kinase deficiency.[provided by RefSeq Apr 2010]

Gene Symbol:   DOLK
Gene Name:   dolichol kinase
Aliases:   CDG1M, DK, DK1, KIAA1094, SEC59, TMEM15
RefSeq:   NC_000009.11 NG_017009.1 NT_008470.19
Ensembl:   ENSG00000175283
Entrez:   22845
UniGene:   Hs.531563
Chromosome Mapping:   9q34.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.780000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download