PrimePCR™ Probe Assay: RAI2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024070
Assay Design:   Exonic
Chromosome Location:   X:17818791-17818967question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000331511 ENST00000360011 ENST00000545871 ENST00000451717 ENST00000415486

Gene Information

Retinoic acid plays a critical role in development cellular growth and differentiation. The specific function of this retinoic acid-induced gene has not yet been determined but it may play a role in development. The chromosomal location of this gene designates it to be a candidate for diseases such as Nance-Horan syndrome sensorineural deafness non-specific X-linked mental retardation oral-facial-digital syndrome and Fried syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq Feb 2010]

Gene Symbol:   RAI2
Gene Name:   retinoic acid induced 2
Aliases:   Not Available
RefSeq:   NC_000023.10 NT_167197.1 NG_016739.1
Ensembl:   ENSG00000131831
Entrez:   10742
UniGene:   Hs.446680
Chromosome Mapping:   Xp22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.210000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download