PrimePCR™ Probe Assay: GH2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GH2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024043
Assay Design:   Exonic
Chromosome Location:   17:61957748-61957876question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000456543 ENST00000423893 ENST00000449787 ENST00000332800

Gene Information

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene along with four other related genes is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones leading to further diversity and potential for specialization. As in the case of its pituitary counterpart growth hormone 1 the predominant isoform of this particular family member shows similar somatogenic activity with reduced lactogenic activity. Mutations in this gene lead to placental growth hormone/lactogen deficiency. [provided by RefSeq Jul 2008]

Gene Symbol:   GH2
Gene Name:   growth hormone 2
Aliases:   GH-V, GHL, GHV, hGH-V
RefSeq:   NC_000017.10 NG_001334.1 NT_010783.15
Ensembl:   ENSG00000136487
Entrez:   2689
Chromosome Mapping:   17q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.860000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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