PrimePCR™ Probe Assay: SLC20A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024009
Assay Design:   Exonic
Chromosome Location:   2:113417079-113417200question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000272542 ENST00000409095

Gene Information

The protein encoded by this gene is a sodium-phosphate symporter that absorbs phosphate from interstitial fluid for use in cellular functions such as metabolism signal transduction and nucleic acid and lipid synthesis. The encoded protein is also a retroviral receptor causing human cells to be susceptible to infection by gibbon ape leukemia virus simian sarcoma-associated virus feline leukemia virus subgroup B and 10A1 murine leukemia virus.[provided by RefSeq Mar 2011]

Gene Symbol:   SLC20A1
Gene Name:   solute carrier family 20 (phosphate transporter), member 1
Aliases:   DKFZp686J2397, FLJ41426, GLVR1, Glvr-1, PIT1, PiT-1
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000144136
Entrez:   6574
UniGene:   Hs.187946
Chromosome Mapping:   2q11-q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.650000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download