This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SLC20A1, Human
PrimePCR™ Template for Probe Assay: SLC20A1, Human
The protein encoded by this gene is a sodium-phosphate symporter that absorbs phosphate from interstitial fluid for use in cellular functions such as metabolism signal transduction and nucleic acid and lipid synthesis. The encoded protein is also a retroviral receptor causing human cells to be susceptible to infection by gibbon ape leukemia virus simian sarcoma-associated virus feline leukemia virus subgroup B and 10A1 murine leukemia virus.[provided by RefSeq Mar 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.