PrimePCR™ Probe Assay: ING4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ING4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ING4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0023951
Assay Design:   Exonic
Chromosome Location:   12:6762429-6762542question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000341550 ENST00000396807 ENST00000446105 ENST00000444704 ENST00000423703 ENST00000412586

Gene Information

This gene encodes a tumor suppressor protein that contains a PHD-finger which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300 a component of the histone acetyl transferase complex suggesting its involvement in the TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed that encode distinct proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   ING4
Gene Name:   inhibitor of growth family, member 4
Aliases:   MGC12557, my036, p29ING4
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000111653
Entrez:   51147
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.110000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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