This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: COL2A1, Human
PrimePCR™ Template for Probe Assay: COL2A1, Human
This gene encodes the alpha-1 chain of type II collagen a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis chondrodysplasia early onset familial osteoarthritis SED congenita Langer-Saldino achondrogenesis Kniest dysplasia Stickler syndrome type I and spondyloepimetaphyseal dysplasia Strudwick type. In addition defects in processing chondrocalcin a calcium binding protein that is the C-propeptide of this collagen molecule are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.