PrimePCR™ Probe Assay: PLEKHH3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0023928
Assay Design:   Exonic
Chromosome Location:   17:40828835-40828948question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000293349 ENST00000412503

Gene Information

Description Not Available

Gene Symbol:   PLEKHH3
Gene Name:   pleckstrin homology domain containing, family H (with MyTH4 domain) member 3
Aliases:   FLJ21019
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000068137
Entrez:   79990
UniGene:   Hs.632251
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.850000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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