PrimePCR™ Probe Assay: TRIM45, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0023925
Assay Design:   Exonic
Chromosome Location:   1:117660820-117660932question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000256649 ENST00000369464 ENST00000369461

Gene Information

This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq Mar 2009]

Gene Symbol:   TRIM45
Gene Name:   tripartite motif containing 45
Aliases:   FLJ13181, RNF99
RefSeq:   NC_000001.10 NT_032977.9 NG_028008.1
Ensembl:   ENSG00000134253
Entrez:   80263
UniGene:   Hs.301526
Chromosome Mapping:   1p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.330000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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