This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0058517
PrimePCR™ PreAmp for SYBR® Green Assay: CCDC140, Human
PrimePCR™ Template for SYBR® Green Assay: CCDC140, Human
This gene encodes a protein that appears to be restricted to select higher primate species. This protein contains a C-terminal coiled-coil domain which is a versatile structural motif consisting of multiple amphipathic alpha-helices that twist around each other to form a supercoil. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.