PrimePCR™ SYBR® Green Assay: P2RY6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best negative control; Same primer pair as used in probe assay qHsaCEP0058497

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057392
Assay Design:   exonic
Chromosome Location:   11:73007753-73007855question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000540342 ENST00000542092 ENST00000349767 ENST00000393592 ENST00000544437 ENST00000393591 ENST00000540124 ENST00000393590 ENST00000535931 ENST00000538328

Gene Information

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity which overlaps in some cases for various adenosine and uridine nucleotides. This receptor is responsive to UDP partially responsive to UTP and ADP and not responsive to ATP. Four transcript variants encoding the same isoform have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   P2RY6
Gene Name:   pyrimidinergic receptor P2Y, G-protein coupled, 6
Aliases:   MGC15335, P2Y6
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000171631
Entrez:   5031
UniGene:   Hs.16362
Chromosome Mapping:   11q13.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.680000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download