PrimePCR™ SYBR® Green Assay: NFE2L1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058455

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NFE2L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NFE2L1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057350
Assay Design:   exonic
Chromosome Location:   17:46135914-46136055question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000362042 ENST00000585291 ENST00000357480 ENST00000361665 ENST00000582155 ENST00000583378 ENST00000536222

Gene Information

This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene NFE2L1 and for "nuclear respiratory factor 1" which has an official symbol of NRF1. [provided by RefSeq Jul 2008]

Gene Symbol:   NFE2L1
Gene Name:   nuclear factor (erythroid-derived 2)-like 1
Aliases:   FLJ00380, LCR-F1, NRF1, TCF11
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000082641
Entrez:   4779
Chromosome Mapping:   17q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.630000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download