This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0058448
PrimePCR™ PreAmp for SYBR® Green Assay: BIK, Human
PrimePCR™ Template for SYBR® Green Assay: BIK, Human
The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins such as BID BAK BAD and BAX that is required for its pro-apoptotic activity and for interaction with anti-apoptotic members of the BCL2 family and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.