PrimePCR™ SYBR® Green Assay: RHOF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058423

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057318
Assay Design:   exonic
Chromosome Location:   12:122231143-122231501question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000267205 ENST00000535560 ENST00000286301 ENST00000379442 ENST00000536621 ENST00000370401 ENST00000432680 ENST00000262858 ENST00000426613 ENST00000284548 ENST00000570156 ENST00000441106 ENST00000422127 ENST00000366707 ENST00000366709 ENST00000599144 ENST00000596765 ENST00000600022 ENST00000593922 ENST00000599223 ENST00000597198 ENST00000377139 ENST00000601291 ENST00000596822 ENST00000600911 ENST00000309877 ENST00000349945 ENST00000432919 ENST00000566899 ENST00000393742 ENST00000567239 ENST

Gene Information

Description Not Available

Gene Symbol:   RHOF
Gene Name:   ras homolog gene family, member F (in filopodia)
Aliases:   ARHF, FLJ20247, RIF
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000139725
Entrez:   54509
UniGene:   Hs.720066
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.310000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download