PrimePCR™ SYBR® Green Assay: PRIM1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058395

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRIM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRIM1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057290
Assay Design:   exonic
Chromosome Location:   12:57139940-57140633question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000338193 ENST00000550770

Gene Information

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus in which DNA polymerase alpha and primase are two key enzymatic components. Primase which is a heterodimer of a small subunit and a large subunit synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small 49 kDa primase subunit. [provided by RefSeq Jul 2008]

Gene Symbol:   PRIM1
Gene Name:   primase, DNA, polypeptide 1 (49kDa)
Aliases:   MGC12308, p49
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000198056
Entrez:   5557
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.870000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download