PrimePCR™ SYBR® Green Assay: MAP9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058381

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057276
Assay Design:   exonic
Chromosome Location:   4:156294378-156294604question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000311277 ENST00000515654 ENST00000433024 ENST00000379248

Gene Information

ASAP is a microtubule-associated protein required for spindle function mitotic progression and cytokinesis (Saffin et al. 2005 [PubMed 16049101]).[supplied by OMIM Mar 2008]

Gene Symbol:   MAP9
Gene Name:   microtubule-associated protein 9
Aliases:   ASAP, FLJ21159
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164114
Entrez:   79884
UniGene:   Hs.61271
Chromosome Mapping:   4q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 36.110000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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