PrimePCR™ SYBR® Green Assay: TRPM3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058303

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRPM3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRPM3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057198
Assay Design:   exonic
Chromosome Location:   9:73461341-73461485question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000377111 ENST00000377110 ENST00000357533 ENST00000396280 ENST00000396285 ENST00000396292 ENST00000358082 ENST00000408909 ENST00000377101 ENST00000396283 ENST00000361823 ENST00000377097 ENST00000423814 ENST00000377106 ENST00000360823 ENST00000377105

Gene Information

The product of this gene belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation-selective channels important for cellular calcium signaling and homeostasis. The protein encoded by this gene mediates calcium entry and this entry is potentiated by calcium store depletion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   TRPM3
Gene Name:   transient receptor potential cation channel, subfamily M, member 3
Aliases:   GON-2, LTRPC3, MLSN2
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000083067
Entrez:   80036
Chromosome Mapping:   9q21.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.930000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download