PrimePCR™ SYBR® Green Assay: HMGN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058279

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057174
Assay Design:   exonic
Chromosome Location:   21:40714961-40715084question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000379441 ENST00000551812 ENST00000549884 ENST00000179765 ENST00000380749 ENST00000380748 ENST00000380747 ENST00000361263

Gene Information

The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein HMG17 the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq Aug 2011]

Gene Symbol:   HMGN1
Gene Name:   high mobility group nucleosome binding domain 1
Aliases:   FLJ27265, FLJ31471, HMG14, MGC104230, MGC117425
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000205581
Entrez:   3150
UniGene:   Hs.356285
Chromosome Mapping:   21q22.3,21q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download