PrimePCR™ SYBR® Green Assay: KSR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058256

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KSR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KSR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057151
Assay Design:   exonic
Chromosome Location:   17:25944360-25948970question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000407242 ENST00000404995 ENST00000260061 ENST00000398988 ENST00000398982 ENST00000582410 ENST00000580430 ENST00000319524 ENST00000509603 ENST00000268763 ENST00000266987 ENST00000456234 ENST00000394357 ENST00000550407 ENST00000527525 ENST00000394227 ENST00000294360 ENST00000360221 ENST00000409399 ENST00000409103 ENST00000421990 ENST00000256997 ENST00000529444 ENST00000527256 ENST00000533929 ENST00000529663 ENST00000537863 ENST00000427569 ENST00000253055 ENST00000372676 ENST00000339946 ENST

Gene Information

Description Not Available

Gene Symbol:   KSR1
Gene Name:   kinase suppressor of ras 1
Aliases:   KSR, RSU2
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000141068
Entrez:   8844
Chromosome Mapping:   17q11.1-q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.260000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download