PrimePCR™ SYBR® Green Assay: URGCP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058245

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: URGCP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: URGCP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057140
Assay Design:   exonic
Chromosome Location:   7:43918646-43918748question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000336086 ENST00000402306 ENST00000443736 ENST00000453200 ENST00000426198 ENST00000455877 ENST00000223341 ENST00000447717

Gene Information

URG4 is upregulated in the presence of hepatitis B virus (HBV)-encoded X antigen (HBxAg) and may contribute to the development of hepatocellular carcinoma by promoting hepatocellular growth and survival (Tufan et al. 2002 [PubMed 12082552]).[supplied by OMIM Mar 2008]

Gene Symbol:   URGCP
Gene Name:   upregulator of cell proliferation
Aliases:   DKFZp666G166, DKFZp686O0457, URG4
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000106608
Entrez:   55665
Chromosome Mapping:   7p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download