PrimePCR™ SYBR® Green Assay: ADAMTSL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0058234

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ADAMTSL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ADAMTSL2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057129
Assay Design:   exonic
Chromosome Location:   9:136412170-136412314question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000354484 ENST00000393061 ENST00000393060

Gene Information

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) and ADAMTS-like protein family. Members of the family share several distinct protein modules including a propeptide region a metalloproteinase domain a disintegrin-like domain and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs and some have unique C-terminal domains. The protein encoded by this gene lacks the protease domain and is therefore of a member of the the ADAMTS-like protein subfamily. It is a secreted glycoprotein that binds the cell surface and extracellular matrix; it also interacts with latent transforming growth factor beta binding protein 1. Mutations in this gene have been associated with geleophysic dysplasia. [provided by RefSeq Feb 2009]

Gene Symbol:   ADAMTSL2
Gene Name:   ADAMTS-like 2
Aliases:   FLJ45164, KIAA0605
RefSeq:   NC_000009.11 NG_009931.1 NT_035014.4
Ensembl:   ENSG00000197859
Entrez:   9719
Chromosome Mapping:   9q34.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.970000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download