This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0058166
PrimePCR™ PreAmp for SYBR® Green Assay: GSK3B, Human
PrimePCR™ Template for SYBR® Green Assay: GSK3B, Human
The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism neuronal cell development and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Sep 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.