PrimePCR™ SYBR® Green Assay: EEF1D, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057981

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056876
Assay Design:   exonic
Chromosome Location:   8:144662816-144663248question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000530109 ENST00000532741 ENST00000526838 ENST00000442189 ENST00000528610 ENST00000395119 ENST00000529272 ENST00000531621 ENST00000524624 ENST00000534380 ENST00000534377 ENST00000533204 ENST00000530191 ENST00000531218 ENST00000533494 ENST00000530445 ENST00000529516 ENST00000423316 ENST00000419152 ENST00000317198

Gene Information

This gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit delta functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1 6 7 9 11 13 17 19.[provided by RefSeq Aug 2010]

Gene Symbol:   EEF1D
Gene Name:   eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Aliases:   EF-1D, EF1D, FLJ20897, FP1047
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000104529
Entrez:   1936
UniGene:   Hs.703306
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.810000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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