This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0057979
PrimePCR™ PreAmp for SYBR® Green Assay: POLR2J3, Human
PrimePCR™ Template for SYBR® Green Assay: POLR2J3, Human
This gene is a member of the RNA polymerase II subunit 11 gene family which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family DNA directed RNA polymerase II polypeptide J has been shown to encode a subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.