PrimePCR™ SYBR® Green Assay: FLYWCH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057971

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FLYWCH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FLYWCH1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056866
Assay Design:   exonic
Chromosome Location:   16:2979687-2979815question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000262953 ENST00000591529 ENST00000443826 ENST00000590536 ENST00000455444 ENST00000426948 ENST00000369609 ENST00000369607 ENST00000369606 ENST00000470142 ENST00000393549 ENST00000455588 ENST00000369601 ENST00000372733 ENST00000537976 ENST00000396958 ENST00000293981 ENST00000573965 ENST00000572006 ENST00000253928 ENST00000416288 ENST00000570425 ENST00000573525 ENST00000399667 ENST00000398148 ENST00000594239

Gene Information

Description Not Available

Gene Symbol:   FLYWCH1
Gene Name:   FLYWCH-type zinc finger 1
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000059122
Entrez:   84256
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.380000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download