PrimePCR™ SYBR® Green Assay: LBX2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057967

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LBX2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LBX2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056862
Assay Design:   exonic
Chromosome Location:   2:74730353-74730449question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000460508 ENST00000395629 ENST00000359120 ENST00000600140 ENST00000457710 ENST00000361200 ENST00000271385 ENST00000443333 ENST00000301067 ENST00000477922 ENST00000302177 ENST00000367607 ENST00000367302 ENST00000367296 ENST00000367295 ENST00000295624 ENST00000367297 ENST00000367300 ENST00000358136 ENST00000356315 ENST00000382192 ENST00000219091 ENST00000318445 ENST00000531499 ENST00000238699 ENST00000378910 ENST00000353632 ENST00000359246 ENST00000247087 ENST00000374011 ENST00000570144 ENST

Gene Information

Description Not Available

Gene Symbol:   LBX2
Gene Name:   ladybird homeobox 2
Aliases:   Not Available
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000179528
Entrez:   85474
Chromosome Mapping:   2p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.740000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download