PrimePCR™ SYBR® Green Assay: KRTAP9-1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057962

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056857
Assay Design:   exonic
Chromosome Location:   17:39346648-39346745question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000398470 ENST00000377721 ENST00000334109 ENST00000391355 ENST00000391354 ENST00000571104 ENST00000570861 ENST00000576511 ENST00000573531 ENST00000568193 ENST00000564962 ENST00000565557 ENST00000565080 ENST00000561589 ENST00000561919 ENST00000567302 ENST00000565268 ENST00000409762 ENST00000409582 ENST00000429174 ENST00000413539 ENST00000258104 ENST00000409651 ENST00000394120 ENST00000409744 ENST00000409366 ENST00000410020 ENST00000410041 ENST00000381222 ENST00000381223 ENST00000329905 ENST

Gene Information

Description Not Available

Gene Symbol:   KRTAP9-1
Gene Name:   keratin associated protein 9-1
Aliases:   KAP9.1, KRTAP9L3
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000240542
Entrez:   728318
UniGene:   Hs.727478
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.992800
y-intercept 37.780000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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