PrimePCR™ SYBR® Green Assay: POR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057953

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056848
Assay Design:   exonic
Chromosome Location:   7:75615675-75615780question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000343933 ENST00000375077 ENST00000461988 ENST00000394893 ENST00000447222 ENST00000439269 ENST00000545601 ENST00000450476 ENST00000419840 ENST00000573555 ENST00000597908 ENST00000597647 ENST00000593343 ENST00000595553 ENST00000593927 ENST00000595759

Gene Information

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors FAD and FMN which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases including apparent combined P450C17 and P450C21 deficiency amenorrhea and disordered steroidogenesis congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   POR
Gene Name:   P450 (cytochrome) oxidoreductase
Aliases:   CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R
RefSeq:   NC_000007.13 NG_008930.1 NT_007933.15
Ensembl:   ENSG00000127948
Entrez:   5447
UniGene:   Hs.354056
Chromosome Mapping:   7q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.710000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download