PrimePCR™ SYBR® Green Assay: SCAND1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057939

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: SCAND1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SCAND1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056834
Assay Design:   exonic
Chromosome Location:   20:34541996-34542194question
Amplicon Length:   169
Splice Variants Targeted:   ENST00000569785 ENST00000413360 ENST00000273582 ENST00000305978 ENST00000373991 ENST00000264832 ENST00000328333 ENST00000454817 ENST00000306749 ENST00000594202 ENST00000596536 ENST00000539407 ENST00000414982 ENST00000600737 ENST00000576346 ENST00000407967 ENST00000319176 ENST00000392549 ENST00000599174 ENST00000316793 ENST00000450053 ENST00000383740 ENST00000586421 ENST00000566228 ENST00000306030 ENST00000508939 ENST00000397501 ENST00000346049 ENST00000517339 ENST00000338238 ENST00000544172 ENST

Gene Information

Description Not Available

Gene Symbol:   SCAND1
Gene Name:   SCAN domain containing 1
Aliases:   RAZ1, SDP1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000171222
Entrez:   51282
Chromosome Mapping:   20q11.1-q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 41.930000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download