PrimePCR™ SYBR® Green Assay: SERF1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057921

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SERF1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SERF1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056816
Assay Design:   exonic
Chromosome Location:   5:70197656-70197760question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000576191 ENST00000572408 ENST00000573686 ENST00000572521 ENST00000573393 ENST00000573188 ENST00000573668 ENST00000570647 ENST00000392474 ENST00000545538 ENST00000380751 ENST00000380750 ENST00000503931 ENST00000506542 ENST00000515588 ENST00000317633 ENST00000511162 ENST00000354833 ENST00000513436 ENST00000504458 ENST00000507348 ENST00000512868 ENST00000512649 ENST00000377086 ENST00000377081 ENST00000287139

Gene Information

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The duplication region includes both a telomeric and a centromeric copy of this gene. Deletions of this gene the telomeric copy often accompany deletions of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients and so it is thought that this gene may be a modifier of the SMA phenotype. The function of this protein is not known; however it bears low-level homology with the RNA-binding domain of matrin-cyclophilin a protein which colocalizes with small nuclear ribonucleoproteins (snRNPs) and the SMN1 gene product. Alternatively spliced transcripts have been documented but it is unclear whether alternative splicing occurs for both the centromeric and telomeric copies of the gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SERF1A
Gene Name:   small EDRK-rich factor 1A (telomeric)
Aliases:   4F5, FAM2A, H4F5, SERF1, SERF1B, SMAM1
RefSeq:   NC_000005.9 NT_006713.15 NW_003315917.1
Ensembl:   ENSG00000172058
Entrez:   8293
Chromosome Mapping:   5q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.110000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download